Structural Biology Using Electrons and X-rays

Structural Biology Using Electrons and X-rays is the perfect book to provide advanced undergraduates or graduate students with an accessible introduction to the two major diffraction-based techniques of structural biology. While concentrating on electron cryo-microscopy with image analysis, it also includes X-ray crystallography in a coherent survey of fundamental principles. Starting with Fourier transforms and progressing through optics and imaging principles, symmetry and three-dimensional reconstruction theory, Structural Biology Using Electrons and X-rays ends in an account of the latest methods adapted to crystals, helices, viral heads, and single particles.

*Emphasis on common principles underlying all diffraction-based methods

*Thorough grounding in theory requires understanding of only simple algebra

*Visual representations and explanations of challenging content

*Mathematical detail offered in short-course form to parallel the text

Part I: Fourier Transforms

1. Overview

2. Correlations & Convolutions

3. Fourier Fundamentals

4. Digital Fourier Transforms

5. Filters

6. Two-Dimensional FTs

Part II: Optics

7. Microscopy with Rays

8. Waves

9. Wave Imaging

Part III: Further Mathematical Methods/General Aspects

10. Symmetry

11. Statistics & Matrices

12. The Third Dimension

Part IV: Image Analysis/Structure Determination

13. Special Symmetries

14. Crystalline Sheets

15. Helices

16. Icosahedral Particles

17. Unsymmetrical ("Single") Particles

18. Distortion Correction

19. FT Mathematics

20. Elementary Matrices