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CELL BIOLOGY ASSAYS
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  • CELL BIOLOGY ASSAYS
ID: 170943
Kreitzer Geri, Julio Celis
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Cell Biology Assays: Protein presents a comprehensive overview of protocols essential for research in proteins.  Chapters provide clear explanations, step-by-step instruction, tips and potential pitfalls.  The important steps and results are illustrated for further ease of use. This text enables researchers at various career levels a plethera of insight into technologies and model systems in biological problem solving.


  • Focus on protein identifcation using mass spectrometry

  • Step-by-step procedures detailing materials, procedures, comment and pitfalls

  • Information on the plethora of technologies needed to tackle complex problems



  1. Protein Determination

  2. Phosphopeptide Mapping: A Basic Protocol

  3. Coupling of Fluorescent Tags to Proteins

  4. Radioiodination of Proteins and Peptides

  5. Free-Flow Electrophoresis

  6. Gel-Based Proteomics: High-Resolution Two-Dimensional Gel Electrophoresis of Proteins. Isoelectric Focusing and Nonequalibrium pH Gradient Electrophoresis.

  7. High-Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients for Proteome Analysis

  8. Two-Dimensional Difference Gel Electrophoresis: Application for the Analysis of Differential Protein Expression in Multiple Biological Samples

  9. Affinity Electrophoresis for Studies of Biospecific Interations: High-Resolution Two-Dimensional Affinity Electrophoresis for Separation of Hapten-Specific Polyclonal Antibodies into Monoclonal Antibodies in Murine Blood Plasma

  10. Image Analysis and Quantitation

  11. Fluorescence Detection of Proteins in Gels Using SYPRO Dyes

  12. Autoradiography and Fluorography: Film-Based Techniques for Imaging Radioactivity in Flat Samples

  13. Two-Dimensional Gel Profiling of Posttanslationally Modified Proteins by in vivo Isotope Labeling

  14. Immunoprecipitation of Proteins under Nondenaturing Conditions

  15. Nondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein Interations: Applications in the Analysis of Mitochrondrial Oxidative Phosphorylation Complexes

  16. Affinity purification with Natural Immobilized Ligands

  17. Analysis of Protein-Protein Interactions by Chemical Cross-Linking

  18. Peroxisomal Targeting as a Tool to Assess Protein-Protein Interactions

  19. Biomolecular Interaction Analysis Mass Spectrometry

  20. Blot Overlays with P-Labedled GST-Ras Fusion Proteins: Application to Mapping Protein-Protein Interaction Sites

  21. Ligand Blot Overlay Assay: Detetion of Ca+2- and Small GTP-Binding Proteins

  22. Modular Scale Yeast Two-Hybrid Screening

  23. Chromophore-Assisted Laser Inactivation of Proteins by Antibodies Labeled with Malachite Green

  24. Chromatin Immunoprecipitation (ChIP)

  25. Gel Mobility Shift Assay

  26. DNA Affinity Chromatography of Transcription Factors: The Oligaonucleotide Trapping Approach

  27. Protein Degradation Methods: Chaperone-Mediated Autophagy

  28. Methods in Protein Ubiquitination

  29. Protein Identification and Sequencing by Mass Spectrometry

  30. Proteome Specific Sample Preparation Methods for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

  31. In-Gel Digestion of Protein Spots for Mass Spectrometry

  32. Peptide Sequencing by Tandem Mass Spectrometry

  33. Direct Database Searing Using Tandem Mass Spectra of Peptides

  34. Identification of Proteins from Organisms with Unsequenced Genomes by Tandem Mass Spectrometry and Sequence-Similarity Database Searching Tools

  35. Identification of Protein Phosphorylation Sites by Mass Spectrometry

  36. Analysis of Carbohydrates/Glycoproteins by Mass Spectrometry

  37. Stable Isotope Labeling by Amindo Acids in Cell Culture for Quantitative Proteomics

  38. Site-Specific, Stable Isotopic Labeling of Cysteinyl Pepties in Complex Peptide Mixtures

  39. Protein Hydrogen Exchange measured by Electrospry Ionization Mass Spectrometry

  40. Nongel Based Proteomics: Selective Reversed-Phase Chromatographic Isolation of Methionine-Containing Peptides from Complex Peptide Mixtures

  41. Mass Spectrometry in Noncovalent Protein Interactions and Protein Assemblies
170943

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