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The Encyclopedia of Mass Spectrometry
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  • The Encyclopedia of Mass Spectrometry
ID: 177130
Richard M. Caprioli, Michael L. Gross
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Volume 2: Biological Applications Part A


The focus of Volume 2 is peptides and proteins. The organization emphasizes the separation techniques, preparation protocols, and foundations of the ionic gas-phase species of biological importance. This volume is divided into four sections: (1) experimental approaches and protocols, (2) sequence analysis, (3) other structural analyses, and (4) targeted applications.


The first section encompass separation procedures (eg, 2-D gel electrophoresis), sample preparation (eg, desalting and enzyme digestion), and instrumentation issues (eg, high resolving power, molecular weight, protein chips, and quantification). H / D exchange, analysis of membrane proteins, and bioinformatics are included.


The next section on the high energy and low energy CAD, protein identification, fundamentals of peptide fragmentation, bottom-up and top-down strategies, chemical derivatization, and post-source decay with MALDI. A section on structure, acoustics, protein-ligand complexes, disulfide analysis, phosphopeptides and phosphoproteins, selenoproteins, nitrated proteins, metal ion binding, and oxidized proteins. Last 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6.. Last 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6. 6..



* Frontier Research on biological, biochemical, and biomedical applications of MS
* Focus on Peptides and Proteins
* Suitable for new graduate students entering the field

CE / MS, analysis by (A. Moseley).

Chemical modification / damage (Steve Barnes).

Derivatization for Analysis (B. Griffiths).

Desalting / purification / microdialysis (Terry Lee).

Disulfide Bond Analysis (Watson).

Enzyme kinetics (Dan Farbris).

Folding / Unfolding (D. Smith).

Fragmentation Mechanisms of Ions (Wesdemiotis).

Gel Electrophoresis (R. Loo).

Glycoproteins (J Peter Katalinic).

H / D Exchange Methods (D. Smith).

High-resolution Measurements by FTMS (Troy Wood).

High Sensitivity Detection (G. Valaskovic).

Hydrolysis, Chemical & Enzymatics (Eckard Nordhoff).

Image Analysis of Proteins in Tissues (Caprioli / Chaurand).

Protein Complexes (A. Link).

LC / MS.

Membrane proteins (Falick).

Metal Ion Binding (Baldwin).

Molecular Weight Measurements (Kondrat).

Neuropeptides (Nyberg).

Non-covalent Interactions, Ligand / Protein (Loo).

Non-covalent Interactions, Protein / Protein (Heck).

Phosphorylation, sulfation.

Proteomics (Jim Carroll).

Quantitation / Proteins.

Quaternary Structure (Terry Farmer).

Secondary Structure.

Sequence Analysis - Digestion / Cleavage (Grant).

Sequence Analysis - High Energy MS / MS (Papayannopoulos).

Sequence Analysis - Low Energy MS / MS (Shabanowitz / Hunt).

Sequence Analysis - PSD with MALDI (Chaurand / Spengler).

Single Cell, analysis of (Sweedler).
177130

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